Asante suffered an embarrassing 3-0 defeat to Tunisian side Etoile du Sahel in Monastir on Sunday evening.
The result ensured the Porcupine Warriors got bundled out of the CAF Champions League 3-2 on aggregate.
Kotoko were trailing 2-0 when Etoile du Sahel had a man sent off but they could not make the numerical advantage count and allowed the Star of Tunisia to score the important third goal.